Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability

Megan R. Miller; Kirstin Frost; Emily L. Smith; Yuhan Jin; Jodie Ulaszek; Matthew Kmet; Karina Hettwer; Anja Schlierf; Sarah M. Nemser; Andriy Tkachenko; Laura B. Goodman; Olgica Ceric; Kelli Almes; Christina Loiacono; Ravinder Reddy; Steffen Uhlig; Gregory H. Tyson

doi:10.1016/j.jfp.2026.100798

Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability

Megan R. Miller
, Kirstin Frost
, Emily L. Smith
, Yuhan Jin
, Jodie Ulaszek
, Matthew Kmet
, Karina Hettwer
, Anja Schlierf
, Sarah M. Nemser
, Andriy Tkachenko
, Laura B. Goodman
, Olgica Ceric
, Kelli Almes
, Christina Loiacono
, Ravinder Reddy
, Steffen Uhlig
, Gregory H. Tyson

Research output: Contribution to journal › Article › peer-review

Original language	English
Article number	100798
Journal	Journal of food protection
Volume	89
Issue number	6
DOIs	https://doi.org/10.1016/j.jfp.2026.100798
State	Published - Jun 2026
Externally published	Yes

ASJC Scopus Subject Areas

Food Science
Microbiology

Keywords

H5N1
Interlaboratory comparison
Milk
rRT-qPCR

Access to Document

10.1016/j.jfp.2026.100798

Cite this

Miller, M. R., Frost, K., Smith, E. L., Jin, Y., Ulaszek, J., Kmet, M., Hettwer, K., Schlierf, A., Nemser, S. M., Tkachenko, A., Goodman, L. B., Ceric, O., Almes, K., Loiacono, C., Reddy, R., Uhlig, S., & Tyson, G. H. (2026). Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability. Journal of food protection, 89(6), Article 100798. https://doi.org/10.1016/j.jfp.2026.100798

Miller, MR, Frost, K, Smith, EL, Jin, Y, Ulaszek, J, Kmet, M, Hettwer, K, Schlierf, A, Nemser, SM, Tkachenko, A, Goodman, LB, Ceric, O, Almes, K, Loiacono, C, Reddy, R, Uhlig, S & Tyson, GH 2026, 'Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability', Journal of food protection, vol. 89, no. 6, 100798. https://doi.org/10.1016/j.jfp.2026.100798

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title = "Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability",

keywords = "H5N1, Interlaboratory comparison, Milk, rRT-qPCR",

author = "Miller, \{Megan R.\} and Kirstin Frost and Smith, \{Emily L.\} and Yuhan Jin and Jodie Ulaszek and Matthew Kmet and Karina Hettwer and Anja Schlierf and Nemser, \{Sarah M.\} and Andriy Tkachenko and Goodman, \{Laura B.\} and Olgica Ceric and Kelli Almes and Christina Loiacono and Ravinder Reddy and Steffen Uhlig and Tyson, \{Gregory H.\}",

note = "Publisher Copyright: {\textcopyright} 2026",

year = "2026",

month = jun,

doi = "10.1016/j.jfp.2026.100798",

language = "English",

volume = "89",

journal = "Journal of food protection",

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T1 - Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability

AU - Miller, Megan R.

AU - Frost, Kirstin

AU - Smith, Emily L.

AU - Jin, Yuhan

AU - Ulaszek, Jodie

AU - Kmet, Matthew

AU - Hettwer, Karina

AU - Schlierf, Anja

AU - Nemser, Sarah M.

AU - Tkachenko, Andriy

AU - Goodman, Laura B.

AU - Ceric, Olgica

AU - Almes, Kelli

AU - Loiacono, Christina

AU - Reddy, Ravinder

AU - Uhlig, Steffen

AU - Tyson, Gregory H.

PY - 2026/6

Y1 - 2026/6

KW - H5N1

KW - Interlaboratory comparison

KW - Milk

KW - rRT-qPCR

UR - https://www.scopus.com/pages/publications/105038455719

UR - https://www.scopus.com/pages/publications/105038455719#tab=citedBy

U2 - 10.1016/j.jfp.2026.100798

DO - 10.1016/j.jfp.2026.100798

M3 - Article

C2 - 42103287

AN - SCOPUS:105038455719

SN - 0362-028X

VL - 89

JO - Journal of food protection

JF - Journal of food protection

IS - 6

M1 - 100798

ER -

Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability

ASJC Scopus Subject Areas

Keywords

Access to Document

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